MSDS Name: Methylene Blue Trihydrate
Catalog Numbers: M235-100
Synonyms: Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride, trihydrate; C.I. Basic Blue 9, trihydrate; C.I. 52015, trihydrate; 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride trihydrate; Methylene Blue trihydrate.
Company Identification:
              Fisher Scientific
              1 Reagent Lane
              Fair Lawn, NJ 07410
For information, call: 201-796-7100
Emergency Number: 201-796-7100
For CHEMTREC assistance, call: 800-424-9300
For International CHEMTREC assistance, call: 703-527-3887

Section 4 - First Aid Measures

Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid.
Skin: Get medical aid. Flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse.
Ingestion: If victim is conscious and alert, give 2-4 cupfuls of milk or water. Never give anything by mouth to an unconscious person. Get medical aid.
Inhalation: Remove from exposure to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid. Do NOT use mouth-to-mouth resuscitation.
Notes to Physician: Treat symptomatically and supportively.

Section 5 - Fire Fighting Measures

General Information: As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. This material in sufficient quantity and reduced particle size is capable of creating a dust explosion.
Extinguishing Media: Use extinguishing media most appropriate for the surrounding fire.

Section 6 - Accidental Release Measures

General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks: Vacuum or sweep up material and place into a suitable disposal container. Clean up spills immediately, observing precautions in the Protective Equipment section. Avoid generating dusty conditions. Provide ventilation.

Section 7 - Handling and Storage

Handling: Wash thoroughly after handling. Remove contaminated clothing and wash before reuse. Use with adequate ventilation. Minimize dust generation and accumulation. Avoid contact with eyes, skin, and clothing. Avoid breathing dust.
Storage: Keep container closed when not in use. Store in a cool, dry, well-ventilated area away from incompatible substances.

Section 8 - Exposure Controls, Personal Protection

Engineering Controls: Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use adequate ventilation to keep airborne concentrations low.
Exposure Limits

Chemical Name	ACGIH	NIOSH	OSHA - Final PELs
Methylene Blue	none listed	none listed	none listed
Methylene Blue trihydrate	none listed	none listed	none listed

OSHA Vacated PELs: Methylene Blue: No OSHA Vacated PELs are listed for this chemical. Methylene Blue trihydrate: No OSHA Vacated PELs are listed for this chemical.
Personal Protective Equipment
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166.
Skin: Wear appropriate protective gloves to prevent skin exposure.
Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators: A respiratory protection program that meets OSHA's 29 CFR §1910.134 and ANSI Z88.2 requirements or European Standard EN 149 must be followed whenever workplace conditions warrant a respirator's use.

Section 9 - Physical and Chemical Properties

Physical State: Solid
Appearance: dark green
Odor: odorless
pH: 3-4.5 (1% aq sol)
Vapor Pressure: Negligible.
Vapor Density: 13 (Air=1)
Evaporation Rate:Not available.
Viscosity: Not available.
Boiling Point: Decomposes.
Freezing/Melting Point:190 deg C (dec)
Autoignition Temperature: Not available.
Flash Point: Not applicable.
Decomposition Temperature:190 deg C
NFPA Rating: (estimated) Health: 2; Flammability: 1; Reactivity: 0
Explosion Limits, Lower:Not available.
Upper: Not available.
Solubility: Slightly soluble.
Specific Gravity/Density:1.230
Molecular Formula:C16H18ClN3S.3H2O
Molecular Weight:373.89

Section 10 - Stability and Reactivity

Chemical Stability: Stable at room temperature in closed containers under normal storage and handling conditions.
Conditions to Avoid: Dust generation, excess heat, excess light.
Incompatibilities with Other Materials: Oxidizing agents, reducing agents, bases, dichromates, alkali iodides, caustic alkalis.
Hazardous Decomposition Products: Chlorine, nitrogen oxides, carbon monoxide, oxides of sulfur, carbon dioxide.
Hazardous Polymerization: Will not occur.

Section 11 - Toxicological Information

RTECS#:
CAS# 61-73-4: SO5600000
CAS# 7220-79-3: SP5740000
LD50/LC50:
CAS# 61-73-4:
Oral, mouse: LD50 = 3500 mg/kg;
Oral, rat: LD50 = 1180 mg/kg;
CAS# 7220-79-3:
Carcinogenicity:
CAS# 61-73-4: Not listed by ACGIH, IARC, NIOSH, NTP, or OSHA. CAS# 7220-79-3: Not listed by ACGIH, IARC, NIOSH, NTP, or OSHA.
Epidemiology: No information available.
Teratogenicity: No information available.
Reproductive Effects: Oral, rat: TDLo = 2500 mg/kg (female 1-22 day(s) after conception) Fertility - post-implantation mortality (e.g. dead and/or resorbed implants per total number of implants).
Neurotoxicity: No information available.
Mutagenicity: DNA Adduct: Mouse, Ascites tumor = 70 umol/L.; DNA Adduct: Mammal - species unspecified Lymphocyte = 1830 nmol/L.
Other Studies: No data available.

Section 12 - Ecological Information

Ecotoxicity: No data available. Laboratory adsorption studies determined that Methylene Blue was strongly adsorbed to three different soils. Volatilization of Methylene Blue will not be important from moist or dry soil surfaces. If released to water, Methylene Blue would adsorb to suspended solids and sediment based upon soil adsorption studies. It will be essentially non-volatile from water surfaces. An estimated BCF value of 1.5 suggests that Methylene Blue will not bioconcentrate in aquatic organisms.
Environmental: If released to the atmosphere, Methylene Blue will exist as both vapor and particulate in the ambient atmosphere. Vapor-phase Methylene Blue is degraded in the atmosphere by reaction with photochemically produced hydroxyl radicals with an estimated half-life of about 1.9 hours. Direct photolysis in the environment may also be possible. Particulate-phase Methylene Blue may be physically removed from the air by wet and dry deposition.
Physical: No information available.
Other: No information available.

Section 13 - Disposal Considerations

Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.
RCRA P-Series: None listed.
RCRA U-Series: None listed.

Section 14 - Transport Information

	US DOT	IATA	RID/ADR	IMO	Canada TDG
Shipping Name:	No information available.				No information available.
Hazard Class:
UN Number:
Packing Group:

Section 15 - Regulatory Information

US FEDERAL

TSCA
CAS# 61-73-4 is listed on the TSCA inventory.
CAS# 7220-79-3 is not listed on the TSCA inventory. It is for research and development use only.
Health & Safety Reporting List
None of the chemicals are on the Health & Safety Reporting List.
Chemical Test Rules
None of the chemicals in this product are under a Chemical Test Rule.
Section 12b
None of the chemicals are listed under TSCA Section 12b.
TSCA Significant New Use Rule
None of the chemicals in this material have a SNUR under TSCA.
SARA

Section 302 (RQ)
None of the chemicals in this material have an RQ.
Section 302 (TPQ)
None of the chemicals in this product have a TPQ.
SARA Codes
CAS # 61-73-4: acute, chronic. CAS # 7220-79-3: acute, chronic.
Section 313
No chemicals are reportable under Section 313.
Clean Air Act:
This material does not contain any hazardous air pollutants. This material does not contain any Class 1 Ozone depletors. This material does not contain any Class 2 Ozone depletors.
Clean Water Act:
None of the chemicals in this product are listed as Hazardous Substances under the CWA. None of the chemicals in this product are listed as Priority Pollutants under the CWA. None of the chemicals in this product are listed as Toxic Pollutants under the CWA.
OSHA:
None of the chemicals in this product are considered highly hazardous by OSHA.
STATE
CAS# 61-73-4 is not present on state lists from CA, PA, MN, MA, FL, or NJ.
CAS# 7220-79-3 is not present on state lists from CA, PA, MN, MA, FL, or NJ.
California No Significant Risk Level: None of the chemicals in this product are listed. European/International Regulations
European Labeling in Accordance with EC Directives
Hazard Symbols:
XN
Risk Phrases:
R 22 Harmful if swallowed.

Safety Phrases:
S 26 In case of contact with eyes, rinse immediately
with plenty of water and seek medical advice.
S 36/37/39 Wear suitable protective clothing, gloves
and eye/face protection.

WGK (Water Danger/Protection)
CAS# 61-73-4: 2
CAS# 7220-79-3: No information available.
Canada
CAS# 61-73-4 is listed on Canada's DSL List. CAS# 61-73-4 is listed on Canada's DSL List. CAS# 61-73-4 is listed on Canada's DSL List.

This product has a WHMIS classification of D2A, D2B.
CAS# 61-73-4 is listed on Canada's Ingredient Disclosure List.
CAS# 7220-79-3 is not listed on Canada's Ingredient Disclosure List.
Exposure Limits

Section 16 - Additional Information

MSDS Creation Date: 8/07/1998
Revision #4 Date: 6/25/2001
The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information, and we assume no liability resulting from its use. Users should make their own investigations to determine the suitability of the information for their particular purposes. In no event shall Fisher be liable for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental, consequential or exemplary damages, howsoever arising, even if Fisher has been advised of the possibility of such damages.